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Genetically modified M13 bacteriophage nanonets for enzyme catalysis and recovery

2019

Article

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Enzyme-based biocatalysis exhibits multiple advantages over inorganic catalysts, including the biocompatibility and the unchallenged specificity of enzymes towards their substrate. The recovery and repeated use of enzymes is essential for any realistic application in biotechnology, but is not easily achieved with current strategies. For this purpose, enzymes are often immobilized on inorganic scaffolds, which could entail a reduction of the enzymes’ activity. Here, we show that immobilization to a nano-scaled biological scaffold, a nanonetwork of end-to-end cross-linked M13 bacteriophages, ensures high enzymatic activity and at the same time allows for the simple recovery of the enzymes. The bacteriophages have been genetically engineered to express AviTags at their ends, which permit biotinylation and their specific end-to-end self-assembly while allowing space on the major coat protein for enzyme coupling. We demonstrate that the phages form nanonetwork structures and that these so-called nanonets remain highly active even after re-using the nanonets multiple times in a flow-through reactor.

Author(s): Kadiri, Vincent Mauricio and Alarcon-Correa, Mariana and Guenther, Jan Philipp and Ruppert, Jacqueline and Bill, Joachim and Rothenstein, Dirk and Fischer, Peer;
Journal: Catalysts
Volume: 9
Pages: 723
Year: 2019
Month: August
Day: 27

Department(s): Micro, Nano, and Molecular Systems
Bibtex Type: Article (article)

DOI: 10.3390/catal9090723
URL: https://www.mdpi.com/2073-4344/9/9/723

BibTex

@article{Kadiri2019,
  title = {Genetically modified M13 bacteriophage nanonets for enzyme catalysis and recovery},
  author = {Kadiri, Vincent Mauricio and Alarcon-Correa, Mariana and Guenther, Jan Philipp and Ruppert, Jacqueline and Bill, Joachim and Rothenstein, Dirk and Fischer, Peer;},
  journal = {Catalysts},
  volume = {9},
  pages = {723},
  month = aug,
  year = {2019},
  url = {https://www.mdpi.com/2073-4344/9/9/723},
  month_numeric = {8}
}